Reporter

Part:BBa_J70662:Design

Designed by: Julie Norville   Group: Knight Lab   (2010-12-29)


Short fusion insert test, designed from J70590 to recreate J04430


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

see above


Source

will be made by adding an ligating a one sided insert into J70590 (that has been cut with BsaXI and PCR purified)

next cut with BsaXI and ligate again (will not need to purify)

problem will be if there is not enough left after first purification (unfortunately BsaXI cannot be heat killed)

References